By Superinnovators 25/07/23

A study published in PLOS ONE has introduced a new method for DNA purification using LEGO robots.

This low-cost DIY approach could have implications for the field of molecular biology.

The research, conducted by Dr. Alonzo Garcia and his team, shows how LEGO robots can be programmed to perform gradient-mixing.

This technique involves the gradual mixing of two solutions, a process that is important in DNA purification.

DNA purification is typically a time-consuming and expensive process.

The team’s use of LEGO robots could potentially reduce both the time and cost associated with this procedure.

Side view of the LEGO gradient mixer during (A) its initial position and (B) its horizontal tilting phase. (1) 3D printed centrifuge-tube holder. (2) Spinning motor to rotate the tubes while in horizontal position. (3) Turning servo motor responsible for tilting the tubes horizontally. (4) Large grey gear connecting the two motors with its small gear complement. (5) The scaffold holding the structure together. (6) The LEGO controller for orchestrating the motions of the two motors. The black cables are traced in white for clarity. CREDIT Sentosa et al., 2023, PLOS ONE

The robots, fitted with a programmable logic controller, can be set to perform the gradient-mixing process with a high level of precision and consistency.

The LEGO robots were tested using a DNA purification protocol.

The robots were able to achieve a good yield of purified DNA, comparable to that achieved by commercial purification kits, but at a lower cost.

(A) Preparation of glycerol gradient with LEGO gradient mixer and separation of sample by RZC. (B) Layers of glycerol before mixing; the blue glycerol at the bottom is 45% (v/v) and the clear glycerol at the top is 15% (v/v). (C) Linear glycerol gradient after mixing with LEGO gradient mixer. (D) 140 nm green fluorescent beads on top of the glycerol gradient before RZC. (E) Fluorescent beads after RZC concentrated into a thin layer due to separation from the glycerol gradient. All glycerol solutions were diluted in 1× TAE 12.5 mM MgCl2. CREDIT Sentosa et al., 2023, PLOS ONE

The use of LEGO robots for DNA purification is not only cost-effective but also accessible.

LEGO robots are widely available and relatively easy to programme, making this method potentially accessible to laboratories worldwide.

This study represents a step forward in the field of molecular biology.

The use of LEGO robots for DNA purification could increase access to genetic research and diagnostics.

Dr. Garcia and his team’s approach demonstrates the potential of using unconventional tools in scientific research.

Their work suggests that sometimes, innovative solutions can be found in unexpected places – even in a child’s toy box.

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